ARG20521

anti-Acetylated Lysine antibody [7F8]

anti-Acetylated Lysine antibody [7F8] for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Other

Gene Regulation antibody

Overview

Product Description

Mouse Monoclonal antibody [7F8] recognizes Acetylated Lysine

Tested Reactivity Other
Tested Application ELISA, ICC/IF, IHC, IP, WB
Specificity Detects proteins containing acetylated lysine residues. Does not detect non-acetylated lysine residues.
Host Mouse
Clonality Monoclonal
Clone 7F8
Isotype IgG1
Target Name Acetylated Lysine
Immunogen Acetylated KLH
Conjugation Un-conjugated

Application Instructions

Cross Reactivity Note Species Independent. Bovine albumin and Avian histones have been tested.
Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
IHC1:100
IPAssay-dependent
WB1:200 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine N ε - acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).

1. Yang XJ. (2005). Oncogene. 24:1653-1662.
2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308.
3. Yang XJ. (2004). Bioessays 26:1076-1087.
4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143.
5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
Research Area Gene Regulation antibody

Images (3) Click the Picture to Zoom In

  • ARG20521 anti-Acetylated Lysine antibody [7F8] IHC-P image

    Immunohistochemistry: Bouin's fixed and paraffin-embedded Mouse backskin stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat anti-Mouse (green) at 1:50 for 1 hour at RT.

  • ARG20521 anti-Acetylated Lysine antibody [7F8] WB image

    Western blot: A549 cell lysate stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:200 dilution.

  • ARG20521 anti-Acetylated Lysine antibody [7F8] WB image

    Western blot: Acetylated lysine in 1) 75 ng of Acetylated BSA, 2) Non-acetylated BSA, and 3) Marker. The blots were stained with ARG20521 anti-Acetylated Lysine antibody [7F8] at 1:1000 dilution.