ARG20523

anti-Acetylated Lysine antibody

anti-Acetylated Lysine antibody for ELISA,ICC/IF,Immunoprecipitation,Western blot and Other

Gene Regulation antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes Acetylated Lysine

Tested Reactivity Other
Tested Application ELISA, ICC/IF, IP, WB
Specificity Detects proteins containing acetylated lysine residues. Does not detect non-acetylated lysine residues.
Host Rabbit
Clonality Polyclonal
Target Name Acetylated Lysine
Immunogen Acetylated KLH
Conjugation Un-conjugated

Application Instructions

Cross Reactivity Note Species Independent.
Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1:100
IPAssay-dependent
WB1:250
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).

1. Yang XJ. (2005). Oncogene. 24:1653-1662.
2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308.
3. Yang XJ. (2004). Bioessays 26:1076-1087.
4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143.
5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
6. Chan, H.M. et al. (2001). Nat. Cell Biol. 3: 667- . 674.
7. Martinez-Balbas, M.A. et al. (2000). EMBO J. 19: 662-671.
Research Area Gene Regulation antibody

Images (4) Click the Picture to Zoom In

  • ARG20523 anti-Acetylated Lysine antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 1 hour) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20523 anti-Acetylated Lysine antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20523 anti-Acetylated Lysine antibody. Right: Composite.

  • ARG20523 anti-Acetylated Lysine antibody WB image

    Western blot: acetylated histone from TSA-treated mouse spleen cells, stained with ARG20523 anti-Acetylated Lysine antibody.

  • ARG20523 anti-Acetylated Lysine antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 1 hour) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20523 anti-Acetylated Lysine antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain. Middle: ARG20523 anti-Acetylated Lysine antibody. Right: Composite.

  • ARG20523 anti-Acetylated Lysine antibody WB image

    Western blot: acetylated lysine in BSA (Left) and Acetylated BSA (Right) stained with ARG20523 anti-Acetylated Lysine antibody at 1:1000 dilution.