ARG52223

anti-Actin antibody [C4]

anti-Actin antibody [C4] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Bovine,C. elegans,Chicken,D. melanogaster ,Dictyostelium discoideum,Human,Mouse,Pig,Rabbit,Rat,Xenopus,Zebrafish

Cancer antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [C4] recognizes Actin
Tested Reactivity Hu, Ms, Rat, Bov, Ce, Chk, D. discoideum, Dm, Pig, Rb, Xenopus, Zfsh
Predict Reactivity Mamm, Vt
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone C4
Isotype IgG1
Target Name Actin
Antigen Species Chicken
Immunogen Preparation of chicken gizzard actin
Conjugation Un-conjugated
Alternate Names CFTDM; MPFD; CFTD; ASMA; NEM1; NEM2; NEM3; Alpha-actin-1; ACTA; CFTD1; Actin, alpha skeletal muscle

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1:500
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WB1:5,000
Application Note Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Ascites fluid
Buffer Ascites fluid and 0.01 % Sodium azide
Preservative 0.01 % Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100154254 Pig ACTA1

GeneID: 11459 Mouse ACTA1

GeneID: 281592 Bovine ACTA1

Gene Symbol ACTA1
Gene Full Name actin, alpha 1, skeletal muscle
Background Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt]
Research Area Cancer antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 42 kDa
PTM Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Images (1) Click the Picture to Zoom In

  • ARG52223 anti-Actin antibody [C4] WB image

    Western blot: Rat hippocampal homogenate showing specific immunolabeling of the ~42 kDa actin protein stained with ARG52223 anti-Actin antibody [C4].

Clone References

Deteriorated stress response in stationary-phase yeast: Sir2 and Yap1 are essential for Hsf1 activation by heat shock and oxidative stress, respectively.

WB / Saccharomyces cerevisiae

Nussbaum I et al.
PLoS One.,  (2014)

publication_link

 

hr_line

A functional MiR-124 binding-site polymorphism in IQGAP1 affects human cognitive performance.

WB / Human

Yang L et al.
PLoS One.,  (2014)

publication_link

 

hr_line

myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis.

WB / Mouse

Goljanek-Whysall K et al.
Development.,  (2014)

publication_link

 

hr_line

Identification of genes in toxicity pathways of trinucleotide-repeat RNA in C. elegans.

Garcia SM et al.
Nat Struct Mol Biol.,  (2014)

publication_link

 

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SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion.

ICC/IF / Human

Williams KC et al.
Mol Biol Cell.,  (2014)

publication_link

 

hr_line

Dichloroacetate prevents restenosis in preclinical animal models of vessel injury.

Deuse T et al.
Nature.,  (2014)

publication_link

 

hr_line

Casein kinase 1 regulates Sprouty2 in FGF-ERK signaling.

Yim DG et al.
Oncogene.,  (2015)

publication_link

 

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Altered migratory behavior of interneurons in a model of cortical dysplasia: the influence of elevated GABAA activity.

WB / Ferret

Abbah J et al.
Cereb Cortex.,  (2014)

publication_link

 

hr_line

Overexpression of ABCG1 protein attenuates arteriosclerosis and endothelial dysfunction in atherosclerotic rabbits.

IHC / Rabbit

Münch G et al.
Heart Int.,  (2012)

publication_link

 

hr_line

N-glycosylation gene DPAGT1 is a target of the Wnt/beta-catenin signaling pathway.

ICC/IF / Human

Sengupta PK et al.
J Biol Chem.,  (2010)

publication_link

 

hr_line

Hepatocyte growth factor improves viability after H2O2-induced toxicity in bile duct epithelial cells.

WB / Dog

Arends B et al.
Comp Biochem Physiol C Toxicol Pharmacol.,  (2008)

publication_link

 

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