ARG66382

anti-Actin antibody [SQab18109]

anti-Actin antibody [SQab18109] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and African green monkey,Bovine,Dog,Human,Mouse,Pig,Rat,Zebrafish

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab18109] recognizes Actin
Tested Reactivity Hu, Ms, Rat, AGMK, Bov, Dog, Pig, Zfsh
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab18109
Isotype IgG
Target Name Actin
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human Actin.
Conjugation Un-conjugated
Full Name actin, alpha 1, skeletal muscle
Alternate Names CFTDM; MPFD; CFTD; ASMA; NEM1; NEM2; NEM3; Alpha-actin-1; ACTA; CFTD1; Actin, alpha skeletal muscle

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:800 - 1:1600
WB1:1000 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 42 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100154254 Pig ACTA1

GeneID: 11459 Mouse ACTA1

GeneID: 281592 Bovine ACTA1

Gene Symbol ACTA1
Gene Full Name actin, alpha 1, skeletal muscle
Background Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt]
PTM Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).

Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG66382 anti-Actin antibody [SQab18109] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human colonic adenocarcinoma stained with ARG66382 anti-Actin antibody [SQab18109] at 1:1600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

  • ARG66382 anti-Actin antibody [SQab18109] WB image

    Western blot: 10 µg of Jurkat, HeLa, 293T, A431 and A375 cell lysates stained with ARG66382 anti-Actin antibody [SQab18109] at 1:1000 dilution.

  • ARG66382 anti-Actin antibody [SQab18109] WB image

    Western blot: 10 µg of MDCK, MDBK, Pig brain, Pig heart, Pig kidney, Zebrafish and Cos-7 lysates stained with ARG66382 anti-Actin antibody [SQab18109] at 1:1000 dilution.

  • ARG66382 anti-Actin antibody [SQab18109] WB image

    Western blot: 10 µg of Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain and Rat liver lysates stained with ARG66382 anti-Actin antibody [SQab18109] at 1:1000 dilution.