ARG52217

anti-Adenylate cyclase 3 antibody

anti-Adenylate cyclase 3 antibody for IHC-Frozen sections,Western blot and Human,Mouse,Rat

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Adenylate cyclase 3
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Adenylate cyclase 3
Antigen Species Rat
Immunogen Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
Conjugation Un-conjugated
Alternate Names AC-III; Adenylate cyclase type III; AC3; ATP pyrophosphate-lyase 3; Adenylate cyclase, olfactive type; Adenylyl cyclase 3; EC 4.6.1.1; Adenylate cyclase type 3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-FrAssay-dependent
WB1:1000 - 1:2000
Application Note Specific for the ~160 kDa adenylate cyclase III protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer PBS
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 104111 Mouse ADCY3

GeneID: 109 Human ADCY3

GeneID: 64508 Rat ADCY3

Gene Symbol ADCY3
Gene Full Name adenylate cyclase 3
Background Adenylate cyclase is the enzyme which produces the “second messenger” signaling molecule, cAMP from ATP. Type III adenylate cyclase is localized to the membranes surrounding neuronal cilia. Much is currently unknown about the function of primary cilia in vertebrates, however, recent work has begun to explore their role in neuronal signaling and neurogenesis (Fuchs and Schwark, 2004; Louvi and Grove 2011).
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 129 kDa
PTM Sumoylated. Sumoylation is required for targeting ot olfactory cilia.
N-glycosylated.
Rapidly phosphorylated after stimulation by odorants or forskolin. Phosphorylation by CaMK2 at Ser-1076 down-regulates enzyme activity.

Images (3) Click the Picture to Zoom In

  • ARG52217 anti-Adenylate cyclase 3 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat hippocampus tissue stained with ARG52217 anti-Adenylate cyclase 3 antibody (red) and costained with ARG10750 anti-MeCP2 antibody [5H12] (green). DAPI (blue) for nuclear staining.

    The Adenylate cyclase 3 antibody reveals neuronal cilia while the MeCP2 antibody reveals the nuclei of certain neurons to a variable degree.

  • ARG52217 anti-Adenylate cyclase 3 antibody ICC/IF image

    Immunofluorescence: Cultured Rat neurons and glia showing strong staining of neuronal cilia stained with ARG52217 anti-Adenylate cyclase 3 antibody (red) and axonal and dendritic staining of ARG52218 alpha II spectrin (green) revealing the submembraneous cytoskeleton and DNA (blue).

  • ARG52217 anti-Adenylate cyclase 3 antibody WB image

    Western blot: Non-transfected HEK293 cells and HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length Human Adenylate cyclase 3 cDNA. The blots were stained with ARG52217 anti-Adenylate cyclase 3 antibody (green) at 1:2000 dilution.

    The same blot was simultaneously stained with ARG52320 anti-GAPDH antibody [1D4] (red) at 1:5000 dilution.