ARG10079

anti-Aflatoxin antibody [1C6]

anti-Aflatoxin antibody [1C6] for ELISA and Other

Microbiology and Infectious Disease antibody

Overview

Product Description

Rat Monoclonal antibody [1C6] recognizes Aflatoxin

Tested Reactivity Other
Tested Application ELISA
Specificity The monoclonal antibody [1C6] is reactive to aflatoxin M1 (AFM1) and aflatoxin B1 (AFB1), but not reactive to BSA and other irrelevant antigens by indirect ELISA.
Host Rat
Clonality Monoclonal
Clone 1C6
Isotype IgG2b,k
Target Name Aflatoxin
Immunogen Winstar rats were immunized with aflatoxin M1-BSA conjugate
Conjugation Un-conjugated

Application Instructions

Application Note ELISA: Reactive to aflatoxin M1 (AFM1) and aflatoxin B1 (AFB1)

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Aflatoxin is a highly toxic mycotoxin produces by a few Aspergillus species. Ingestion of aflatoxin contaminated food causes hepatic necrosis, cirrhosis and cancer in human and animal. Exposure to high level aflatoxin results in acute hepatic failure manifested by heamorrhage, edema, mental disorder and coma. Aflatoxin B1, the most toxic isoform, is produced by Aspergillus flavus and Aspergillus parasiticus. Aflatoxin M1 is the metabolic product of B1. M1 is found in the milk of cows that fed on contaminated feed and also in the fermentation broth of Aspergillus parasiticus. Chronic exposure to the toxin in milk and food impedes the normal growth and development in children.
Resrarch Area Microbiology and Infectious Disease antibody

Clone References

Fluorimetric immunoassay for multianalysis of aflatoxins.

ELISA / 

Kanungo L et al.
J Anal Methods Chem.,  (2013)

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Electrochemical immunochip sensor for aflatoxin M1 detection.

Parker CO et al.
Anal Chem.,  (2009)

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Development of an electrochemical immunosensor for aflatoxin M1 in milk with focus on matrix interference.

Parker CO et al.
Biosens Bioelectron.,  (2009)

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