ARG10694

anti-Aldolase C antibody [1A1]

anti-Aldolase C antibody [1A1] for Western blot,ICC/IF,IHC-Frozen sections and Human,Horse,Cow,Pig,Rat,Mouse

Overview

Product Description Mouse Monoclonal antibody [1A1] recognizes Aldolase C
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 1A1
Isotype IgG1
Target Name Aldolase C
Immunogen C-terminal sequence KYEGSGEDGGAAAQSLYIANHAY
Conjugation Un-conjugated
Full Name aldolase C, fructose-bisphosphate
Alternate Names EC 4.1.2.13; Brain-type aldolase; Fructose-bisphosphate aldolase C; ALDC

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 11676 Mouse ALDOC

GeneID: 230 Human ALDOC

GeneID: 24191 Rat ALDOC

Gene Symbol ALDOC
Gene Full Name aldolase C, fructose-bisphosphate
Background This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

Images (3) Click the Picture to Zoom In

  • ARG10694 anti-Aldolase C antibody [1A1] ICC/IF image

    Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10694 anti-Aldolase C antibody [1A1] (green) and co-stained with rabbit antibody to NeuN/FOX3 antibody (RPCA-FOX3, red). MCA-1A1 antibody reveals strong cytoplasmic staining in astrocytes, while Rabbit Fox3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and is complete absence of astrocytes. Blue is a DNA stain.

  • ARG10694 anti-Aldolase C antibody [1A1] WB image

    Western blot: Crude Rat brain lysates stained with ARG10694 anti-Aldolase C antibody [1A1]. Other studies show that this antibody is completely specific for Aldolase C, and does not react with recombinant Human Aldolase A or B.

  • ARG10694 anti-Aldolase C antibody [1A1] WB image

    Western blot: Recombinant Human Aldolase A, B and C with three monoclonal antibodies as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged Human Aldolase A, B and C respectively. The epitope for ARG10694 anti-Aldolase C antibody [1A1] is within the C-terminal peptide of the molecule which is less conserved between the three Aldolases. Clone 4A9 binds to the N-terminal peptide and also is specific for Aldolase C, while clone E9 binds to the less conserved core of the molecule and recognizes all three Aldolase C gene products.