ARG10695

anti-Aldolase C antibody [4A9]

anti-Aldolase C antibody [4A9] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

Overview

Product Description Mouse Monoclonal antibody [4A9] recognizes Aldolase C
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 4A9
Isotype IgG1
Target Name Aldolase C
Immunogen N-terminal sequence MPHSYPALSAEQKKELSDIA
Conjugation Un-conjugated
Full Name aldolase C, fructose-bisphosphate
Alternate Names EC 4.1.2.13; Brain-type aldolase; Fructose-bisphosphate aldolase C; ALDC

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 11676 Mouse ALDOC

GeneID: 230 Human ALDOC

GeneID: 24191 Rat ALDOC

Gene Symbol ALDOC
Gene Full Name aldolase C, fructose-bisphosphate
Background This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

Images (9) Click the Picture to Zoom In

  • ARG10695 anti-Aldolase C antibody [4A9] ICC/IF image

    Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10695 anti-Aldolase C antibody [4A9] (green) and co-stained with our rabbit antibody to FOX3 / NeuN (red). ARG10695 anti-Aldolase C antibody [4A9] antibody reveals strong cytoplasmic staining in astrocytes, while Rabbit FOX3 / NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and is complete absence of astrocytes. Blue is a DNA stain.

  • ARG10695 anti-Aldolase C antibody [4A9] ICC/IF image

    Immunofluorescence: Cortical neuron-glial cells from E20 Rat stained with ARG10695 anti-Aldolase C antibody [4A9] (green) at 1:1000 dilution and costained with ARG52328 anti-MAP2 antibody (red) at 1:10000 dilution. Hoechst (blue) for nuclear staining.

    The Aldolase C antibody labels cytosolic protein expressed in the glial cells, while the MAP2 antibody stains dendrites and perikarya of mature neurons.

  • ARG10695 anti-Aldolase C antibody [4A9] IHC-Fr image

    Immunohistochemistry: Frozen section of Rat cerebellum stained with ARG10695 anti-Aldolase C antibody [4A9] (green) at 1:1000 dilution and costained with ARG52312 anti-GFAP antibody (red) at 1:5000 dilution. Hoechst (blue) for nuclear staining.

    Aldolase C antibody selectively labels the perikarya and dendrites of Purkinje cells, while GFAP antibody stains processes of Bergman glia and astrocytic cells.

  • ARG10695 anti-Aldolase C antibody [4A9] IHC-Fr image

    Immunohistochemistry: Frozen sections of Mouse brain (fixed by transcardial perfusion with 4% paraformaldehyde) stained with ARG10695 anti-Aldolase C antibody [4A9] (red) and co-stained with chicken anti-Vimentin  antibody (green). In the striatum, ARG10695 anti-Aldolase C antibody [4A9] positive astrocytes are highly co-stained with Vimentin  antibody, which results in yellow to gold colors. Insets show a higher magnification picture. Nuclei are labeled with DAPI (blue).

  • ARG10695 anti-Aldolase C antibody [4A9] WB image

    Western blot: Crude cow cerebellum homogenate stained with ARG10695 anti-Aldolase C antibody [4A9]. Other studies show that this antibody does not recognize the very closely related molecules aldolase A or B.

  • ARG10695 anti-Aldolase C antibody [4A9] WB image

    Western blot: Rat brain, Mouse brain, Cow cerebellum and Pig hippocampus lysates stained with ARG10695 anti-Aldolase C antibody [4A9] (green) at 1:2000 dilution.

  • ARG10695 anti-Aldolase C antibody [4A9] IHC-Fr image

    Immunohistochemistry: Frozen section of E20 Rat cortical tissue stained with ARG10695 anti-Aldolase C antibody [4A9] (green) at 1:1000 dilution and costained with ARG52328 anti-MAP2 antibody (red) at 1:10000 dilution.

    The aldolase C antibody labels cytosolic protein expressed in glial cells, while MAP2 antibody stains dendrites and perikarya of mature neurons.

  • ARG10695 anti-Aldolase C antibody [4A9] IHC-Fr image

    Immunohistochemistry: Frozen sections of Mouse brain (fixed by transcardial perfusion with 4% paraformaldehyde) stained with ARG10695 anti-Aldolase C antibody [4A9] (red) and co-stained with chicken anti-Vimentin  antibody (green). In the cerebellum, ARG10695 anti-Aldolase C antibody [4A9] positive Purkinje cells do not express vimentin, which results in red color. Insets show a higher magnification picture. Nuclei are labeled with DAPI (blue).

  • ARG10695 anti-Aldolase C antibody [4A9] WB image

    Western blot: Recombinant Human Aldolase A, B and C with three monoclonal antibodies as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged Human Aldolase A, B and C respectively. The epitope for ARG10695 anti-Aldolase C antibody [4A9] is within the N-terminal peptide of the molecule which is less conserved between the three Aldolases. Clone 1A1 binds to the C-terminal peptide and also is specific for Aldolase C, while clone E9 binds to the less conserved core of the molecule and recognizes all three Aldolase C gene products.