ARG10693

anti-Aldolase antibody [E9]

anti-Aldolase antibody [E9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Cow,Horse,Human,Mouse,Pig,Rat

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anti-Aldolase antibody [E9] Mouse Monoclonal antibody [E9] recognizes Aldolase ARG10693 Arigo biolaboratories
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anti-Aldolase antibody [E9] is rated 5.0 out of 5 by 2.

anti-Aldolase antibody [E9]

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Overview

Product Description Mouse Monoclonal antibody [E9] recognizes Aldolase
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone E9
Isotype IgG1
Target Name Aldolase
Antigen Species Human
Immunogen Recombinant Human full-length Aldolase C.
Conjugation Un-conjugated
Full Name aldolase A, fructose-bisphosphate
Alternate Names Lung cancer antigen NY-LU-1; HEL-S-87p; GSD12; Fructose-bisphosphate aldolase A; Muscle-type aldolase; EC 4.1.2.13; ALDA

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
IHC-PAssay-dependent
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 11674 Mouse ALDOA

GeneID: 226 Human ALDOA

GeneID: 24189 Rat ALDOA

Gene Symbol ALDOA
Gene Full Name aldolase A, fructose-bisphosphate
Background The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]
Function Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity). [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG10693 anti-Aldolase antibody [E9] ICC/IF image

    Immunocytochemistry: Rat mixed neuron / glial cultures stained with ARG10693 anti-Aldolase antibody [E9] (red) and co-stained with chicken antibody to GFAP (green). ARG10693 anti-Aldolase antibody [E9] antibody reveals strong cytoplasmic staining in astrocytes. Blue is a DNA stain. Aldolase stains the astrocytes cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in subset of astrocytes.

  • ARG10693 anti-Aldolase antibody [E9] WB image

    Western blot: Rat brain lysate stained with ARG10693 anti-Aldolase antibody [E9]. This antibody binds to an epitope shared by the closely related Aldolase A, B and C, recognizing all three on WB.

  • ARG10693 anti-Aldolase antibody [E9] WB image

    Western blot: Recombinant Human Aldolase A, B and C with three monoclonal antibodies as indicated showing binding to all three gene products. Lane labelled S show molecular weight standards, while lanes A, B and C contain recombinant full length His-tagged Human Aldolase A, B and C respectively. The epitope for ARG10693 anti-Aldolase antibody [E9] is within the core of the three Aldolase molecules which are less conserved than the N- and C-termini where our Aldolase C specific antibodies bind. Clone 4A9 binds to the N-terminal peptide and clone 1A1 binds to the C-terminal peptide.

Customer's Feedback

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Review for anti-Aldolase antibody [E9]

Application:WB

Sample:293T

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Aldolase antibody [E9]

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload

IHC-P / Rat

Zhang Mingjing et al.
J Cell Mol Med.,  (2018)

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