ARG66207

anti-Androgen Receptor antibody [SQab1729]

anti-Androgen Receptor antibody [SQab1729] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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anti-Androgen Receptor antibody [SQab1729]

Recombinant Rabbit Monoclonal antibody [SQab1729] recognizes Androgen Receptor

ARG66207 Arigo biolaboratories
4.0 out of 5 based on 1
anti-Androgen Receptor antibody [SQab1729] is rated 4.0 out of 5 by 1.

anti-Androgen Receptor antibody [SQab1729]

Overview

Product Description

Recombinant Rabbit Monoclonal antibody [SQab1729] recognizes Androgen Receptor

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1729
Target Name Androgen Receptor
Species Human
Immunogen Synthetic peptide around the N-terminus of Androgen Receptor.
Conjugation Un-conjugated
Full Name androgen receptor
Alternate Names TFM; Dihydrotestosterone receptor; Androgen receptor; KD; AR8; HUMARA; NR3C4; AIS; SBMA; HYSP1; SMAX1; Nuclear receptor subfamily 3 group C member 4; DHTR

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 ‐ 1:200
WB1:2000 ‐ 1:5000
Application Note IHC-P: Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 99 kDa
Observed Size 100 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 11835 Mouse AR

GeneID: 24208 Rat AR

GeneID: 367 Human AR

Gene Symbol AR
Gene Full Name androgen receptor
Background Androgen Receptor is a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
Function Androgen Receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones. [UniProt]
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PTM Sumoylated on Lys-388 (major) and Lys-521. Ubiquitinated. Deubiquitinated by USP26. 'Lys-6' and 'Lys-27'-linked polyubiquitination by RNF6 modulates AR transcriptional activity and specificity.
Phosphorylated in prostate cancer cells in response to several growth factors including EGF. Phosphorylation is induced by c-Src kinase (CSK). Tyr-535 is one of the major phosphorylation sites and an increase in phosphorylation and Src kinase activity is associated with prostate cancer progression. Phosphorylation by TNK2 enhances the DNA-binding and transcriptional activity and may be responsible for androgen-independent progression of prostate cancer. Phosphorylation at Ser-83 by CDK9 regulates AR promoter selectivity and cell growth. Phosphorylation by PAK6 leads to AR-mediated transcription inhibition.
Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.

Images (3) Click the Picture to Zoom In

  • ARG66207 anti-Androgen Receptor antibody [SQab1729] IHC-P image

    Immunohistochemistry: Formalin‐fixed and paraffin‐embedded Human prostate tissue stained with ARG66207 anti-Androgen Receptor antibody [SQab1729] at 1:200 dilution.

    Antigen retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0

  • ARG66207 anti-Androgen Receptor antibody [SQab1729] WB image

    Western blot: 10 µg of T47D and A549 cell lysates stained with ARG66207 anti-Androgen Receptor antibody [SQab1729] at 1:2000 dilution.

  • ARG66207 anti-Androgen Receptor antibody [SQab1729] WB image

    Western blot: 10 µg of Rat testis and Mouse testis lysates stained with ARG66207 anti-Androgen Receptor antibody [SQab1729] at 1:5000 dilution.

Customer's Feedback

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Review for anti-Androgen Receptor antibody [SQab1729]

Application:WB

Sample:Mouse testis

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC