ARG10540

anti-Annexin A1 antibody [ANEX 5E4/1]

anti-Annexin A1 antibody [ANEX 5E4/1] for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human

Signaling Transduction antibody
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anti-Annexin A1 antibody [ANEX 5E4/1]

Mouse Monoclonal antibody [5E4/1] recognizes Annexin A1

ARG10540 Arigo biolaboratories
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anti-Annexin A1 antibody [ANEX 5E4/1] is rated 5.0 out of 5 by 1.

anti-Annexin A1 antibody [ANEX 5E4/1]

Overview

Product Description

Mouse Monoclonal antibody [5E4/1] recognizes Annexin A1

Tested Reactivity Hu
Tested Application ELISA, ICC/IF, IHC, IP, WB
Host Mouse
Clonality Monoclonal
Clone ANEX 5E4/1
Isotype IgG1
Target Name Annexin A1
Antigen Species Human
Immunogen Mixture of native membrane proteins from human bone tissues which includes native whole annexin proteins.
Conjugation Un-conjugated
Full Name annexin A1
Alternate Names ANX1; LPC1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay dependent
ICC/IFAssay dependent
IHCAssay dependent
IP1 - 2 µg/100–500 µg lysate (in 1ml lysate)
WBAssay dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Endothelial cells
Calculated MW 32 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 0.9 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol ANXA1
Gene Full Name annexin A1
Background This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
Function Annexin A1 Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Promotes resolution of inflammation and wound healing (PubMed:25664854). Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). . Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca2+ and can bind up to eight Ca2+ ions (By similarity). Displays Ca2+-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). [provide by Uniprot]
Research Area Signaling Transduction antibody
PTM Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:2457390, PubMed:15485879). Phosphorylated in response to EGF treatment (PubMed:2532504).
Sumoylated.

Images (1) Click the Picture to Zoom In

  • ARG10540 anti-Annexin A1 antibody [ANEX 5E4/1] WB image

    Western blot: 30 µg of HeLa cell lysate stained with ARG10540 anti-Annexin A1 antibody [ANEX 5E4/1] at 1:500 dilution.

Customer's Feedback

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Review for anti-Annexin A1 antibody [ANEX 5E4/1]

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC