ARG52228

anti-Aquaporin 2 phospho (Ser261) antibody

anti-Aquaporin 2 phospho (Ser261) antibody for Western blot,Immunohistochemistry and Rat

Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Aquaporin 2 phospho (Ser261)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Bov, Chk, Dog, NHuPrm
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Aquaporin 2
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 conjugated to KLH
Conjugation Un-conjugated
Alternate Names Aquaporin-2; Aquaporin-CD; AQP-2; ADH water channel; Collecting duct water channel protein; Water channel protein for renal collecting duct; AQP-CD; WCH-CD

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WB1:1000
Application Note Specific for ~29k AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~ 37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25386 Rat AQP2

Swiss-port # P34080 Rat Aquaporin-2

Gene Symbol AQP2
Gene Full Name aquaporin 2 (collecting duct)
Background Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007).
Research Area Metabolism antibody; Signaling Transduction antibody
Calculated MW 29 kDa
PTM Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.

Images (1) Click the Picture to Zoom In

  • ARG52228 anti-Aquaporin 2 phospho (Ser261) antibody WB image

    Western blot: Rat kidney lysate showing phospho-specific immunolabeling of the ~29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser 261 stained with ARG52228 anti-Aquaporin 2 phospho (Ser261) antibody.