ARG10698

anti-Aurora A + B antibody [3H1]

anti-Aurora A + B antibody [3H1] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Pig,Dog,Horse

Overview

Product Description Mouse Monoclonal antibody [3H1] recognizes Aurora A + B
Tested Reactivity Hu, Ms, Rat, Cow, Dog, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 3H1
Isotype IgG1
Target Name Aurora A + B
Antigen Species Human
Immunogen Full length recombinant Human Aurora A protein expressed in and purified from E. coli.
Conjugation Un-conjugated
Full Name aurora kinase A
Alternate Names ARK-1; AIK; BTAK; Serine/threonine-protein kinase 6; Breast tumor-amplified kinase; Serine/threonine-protein kinase aurora-A; STK15; Serine/threonine-protein kinase 15; AURORA2; Aurora-related kinase 1; hARK1; AURA; STK6; STK7; Aurora kinase A; EC 2.7.11.1; Aurora/IPL1-related kinase 1; Aurora 2; ARK1; PPP1R47

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 20878 Mouse AURKA

GeneID: 574063 Pig AURKA

GeneID: 6790 Human AURKA

Gene Symbol AURKA
Gene Full Name aurora kinase A
Background The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. [UniProt]
PTM Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis.
Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome. Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.

Images (4) Click the Picture to Zoom In

  • ARG10698 anti-Aurora A + B antibody [3H1] WB image

    Western blot: Cells were treated with 100 ng/ml of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase, for 6 hours prior western blotting. HeLa, Dog A72, Horse NBL6 and Mouse KR158 cell lysates stained with ARG10698 anti-Aurora A + B antibody [3H1].

  • ARG10698 anti-Aurora A + B antibody [3H1] ICC/IF image

    Immunocytochemistry: HeLa cell cultures were stained with ARG10698 anti-Aurora A + B antibody [3H1] (green). The antibody stains spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were co-stained with a chicken polyclonal antibody to Vimentin  (red). Blue is a DNA stain.

  • ARG10698 anti-Aurora A + B antibody [3H1] WB image

    Western blot: Left: HeLa cells treated with 100 ng/ml nocodazole for 18 hours was stained with ARG10698 anti-Aurora A + B antibody [3H1]. Nocodazole is a microtubule depolymerizing agent which induces cells to halt at the G2/M phase and also induces Aurora A expression.  Right: Recombinant Human Aurora A, B and C were stained with ARG10698, which binds to both Aurora A and B.

  • ARG10698 anti-Aurora A + B antibody [3H1] WB image

    Western blot: Purified full length Human recombinant Aurora A, B, C proteins stained with ARG10698 anti-Aurora A + B antibody [3H1].