ARG57215
anti-B Raf (V600E) antibody [RM8]
anti-B Raf (V600E) antibody [RM8] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Monoclonal antibody [RM8] recognizes B Raf (V600E) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | RM8 |
| Isotype | IgG |
| Target Name | B Raf (V600E) |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from Human BRAF V600E mutant. |
| Conjugation | Un-conjugated |
| Alternate Names | BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol and 1% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf |
|---|---|
| Gene Symbol | BRAF |
| Gene Full Name | B-Raf proto-oncogene, serine/threonine kinase |
| Background | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt] |
| Calculated MW | 84 kDa |
| PTM | Phosphorylation at Ser-365 by SGK1 inhibits its activity. Methylation at Arg-671 decreases stability and kinase activity. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF. |
Images (5) Click the Picture to Zoom In
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ARG57215 anti-B Raf (V600E) antibody [RM8] ICC/IF image
Immunocytochemistry: Human WiDr cells stained with ARG57215 anti-B Raf (V600E) antibody [RM8]. (400X, Positive).
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ARG57215 anti-B Raf (V600E) antibody [RM8] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded melanoma tissue sections stained with ARG57215 anti-B Raf (V600E) antibody [RM8].
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ARG57215 anti-B Raf (V600E) antibody [RM8] WB image
Western blot: Cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein. The blots were stained with ARG57215 anti-B Raf (V600E) antibody [RM8].
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ARG57215 anti-B Raf (V600E) antibody [RM8] ELISA image
Sandwich ELISA: Titration curve of ARG57215 anti-B Raf (V600E) antibody [RM8]. Antigen: Lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins.
His-tag fusion proteins were captured by an anti-His-Tag antibody.
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ARG57215 anti-B Raf (V600E) antibody [RM8] ICC/IF image
Immunocytochemistry: Human SW40 cells stained with ARG57215 anti-B Raf (V600E) antibody [RM8]. (400X, Negative).
