ARG54673

anti-B Raf antibody

anti-B Raf antibody for ELISA,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes B Raf
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, WB
Specificity At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name B Raf
Immunogen Synthetic peptide (19 aa) within aa. 330-380 Human B-raf protein.
Conjugation Un-conjugated
Alternate Names BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
ICC/IFAssay-Dependent
IHCAssay-Dependent
WB1 - 2 μg/mL
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Brain Tissue Lysate
Observed Size 99 kDa. Multiple post-translational modifications, commonly observed at 95-100 kDa.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 109880 Mouse BRAF

GeneID: 673 Human BRAF

Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf

Swiss-port # P28028 Mouse Serine/threonine-protein kinase B-raf

Gene Symbol BRAF 
Gene Full Name B-Raf proto-oncogene, serine/threonine kinase
Background B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance. |
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Calculated MW 84 kDa
PTM Phosphorylation at Ser-365 by SGK1 inhibits its activity.
Methylation at Arg-671 decreases stability and kinase activity.
Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

Images (3) Click the Picture to Zoom In

  • ARG54673 anti-B Raf antibody ICC/IF image

    Immunofluorescence: Human small intestine tissue stained with ARG54673 anti-B Raf antibody at 20 μg/ml.

  • ARG54673 anti-B Raf antibody IHC image

    Immunohistochemistry: Human small intestine tissue stained with ARG54673 anti-B Raf antibody at 2.5 μg/ml.

  • ARG54673 anti-B Raf antibody WB image

    Western blot: Human brain tissue lysate stained with ARG54673 anti-B Raf antibody at (A) 1 and (B) 2 μg/ml.