ARG45600

anti-B3GNT2 antibody

anti-B3GNT2 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes B3GNT2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name B3GNT2
Antigen Species Human
Immunogen Recombinant protein containing to human B3GNT2.
Conjugation Un-conjugated
Alternate Names B3GNT2; UDP-GlcNAc:BetaGal Beta-1,3-N-Acetylglucosaminyltransferase 2; BETA3GNT; B3GNT-2; B3GN-T2; B3GNT1; N-Acetyllactosaminide Beta-1,3-N-Acetylglucosaminyltransferase 2; UDP-GlcNAc:BetaGal Beta-1,3-N-Acetylglucosaminyltransferase 1; Beta3Gn-T1; Beta3Gn-T2; B3GN-T1; BGnT-2; UDP-Galactose:Beta-N-Acetylglucosamine Beta-1,3-Galactosyltransferase 7; UDP-Gal:Beta-GlcNAc Beta-1,3-Galactosyltransferase 7; Beta-1,3-N-Acetylglucosaminyltransferase BGnT-1; Beta-1,3-N-Acetylglucosaminyltransferase BGnT-2; Beta-1,3-N-Acetylglucosaminyltransferase 1; Beta-1,3-N-Acetylglucosaminyltransferase 2; Beta-1,3-Galactosyltransferase 7; Beta-1,3-GalTase 7; Beta-1,3-Gn-T1; Beta-1,3-Gn-T2; Beta-3-Gx-T7; EC 2.4.1.149; Beta3Gal-T7; Beta3GalT7; B3Gal-T7; 3-Gn-T1; 3-Gn-T2; B3GALT7; Beta-1; BGnT-1; B3GNT; BGNT2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 55 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10678 Human B3GNT2

GeneID: 53625 Mouse B3GNT2

Swiss-port # Q9NY97 Human N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2

Swiss-port # Q9Z222 Mouse N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2

Gene Symbol B3GNT2
Gene Full Name UDP-GlcNAc:BetaGal Beta-1,3-N-Acetylglucosaminyltransferase 2
Background This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2016]
Function Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains. Shows a marked preference for Gal(beta1-4)Glc(NAc)-based acceptors. Probably constitutes the main polylactosamine synthase. [UniProt]
Cellular Localization Golgi apparatus; Membrane. [UniProt]
Calculated MW 46 kDa
PTM Glycoprotein. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45600 anti-B3GNT2 antibody ICC/IF image

    Immunofluorescence: MCF-7 stained with ARG45600 anti-B3GNT2 antibody at 5 μg/ml dilution.

  • ARG45600 anti-B3GNT2 antibody WB image

    Western blot: MOLT-4 stained with ARG45600 anti-B3GNT2 antibody at 0.5 μg/ml dilution.

  • ARG45600 anti-B3GNT2 antibody FACS image

    Flow Cytometry: Hela stained with ARG45600 anti-B3GNT2 antibody at 1 µg/10^6 cells dilution.

  • ARG45600 anti-B3GNT2 antibody WB image

    Western blot: Rat brain stained with ARG45600 anti-B3GNT2 antibody at 0.5 μg/ml dilution.

  • ARG45600 anti-B3GNT2 antibody WB image

    Western blot: Mouse brain stained with ARG45600 anti-B3GNT2 antibody at 0.5 μg/ml dilution.