ARG45528
anti-BAZ1A / ACF1 antibody
anti-BAZ1A / ACF1 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BAZ1A / ACF1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BAZ1A / ACF1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human BAZ1A / ACF1. |
| Conjugation | Un-conjugated |
| Alternate Names | BAZ1A; bromodomain adjacent to zinc finger domain, 1A; WCRF180; hWALp1; ACF1; CHRAC subunit ACF1; hACF1; Bromodomain adjacent to zinc finger domain protein 1A; Williams syndrome transcription factor-related chromatin-remodeling factor 180; WALp1; ATP-utilizing chromatin assembly and remodeling factor 1; ATP-dependent chromatin-remodeling protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 203 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NRL2 Human Bromodomain adjacent to zinc finger domain protein 1A |
|---|---|
| Gene Symbol | BAZ1A |
| Gene Full Name | bromodomain adjacent to zinc finger domain, 1A |
| Background | The BAZ1A gene encodes the accessory subunit of the ATP-dependent chromatin assembly factor (ACF), a member of the ISWI ('imitation switch') family of chromatin remodeling complexes (summarized by Racki et al., 2009 [PubMed 20033039]).[supplied by OMIM, Apr 2010] |
| Function | Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression. Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 179 kDa |
| PTM | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45528 anti-BAZ1A / ACF1 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45528 anti-BAZ1A / ACF1 antibody at 5 μg/ml dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody WB image
Western blot: Jurkat stained with ARG45528 anti-BAZ1A / ACF1 antibody at 0.5 μg/ml dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody FACS image
Flow Cytometry: HepG2 stained with ARG45528 anti-BAZ1A / ACF1 antibody at 1 µg/10^6 cells dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody WB image
Western blot: C6 stained with ARG45528 anti-BAZ1A / ACF1 antibody at 0.5 μg/ml dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody WB image
Western blot: Mouse testis stained with ARG45528 anti-BAZ1A / ACF1 antibody at 0.5 μg/ml dilution.
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ARG45528 anti-BAZ1A / ACF1 antibody WB image
Western blot: COS-7 stained with ARG45528 anti-BAZ1A / ACF1 antibody at 0.5 μg/ml dilution.
