ARG10146

anti-BNP antibody [50E1]

anti-BNP antibody [50E1] for ELISA,Western blot and Human

Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [50E1] recognizes BNP
Tested Reactivity Hu
Tested Application ELISA, WB
Specificity Human BNP and proBNP
Host Mouse
Clonality Monoclonal
Clone 50E1
Isotype IgG1
Target Name BNP
Antigen Species Human
Immunogen Human synthetic BNP (whole molecule)
Conjugation Un-conjugated
Full Name natriuretic peptide B
Alternate Names 4-27; Natriuretic peptides B; 1-28; 1-29; 5-32; BNP; 1-32; 1-30; BNP-32; 5-29; 2-31; 4-30; 4-31; 4-32; 3-29; Gamma-brain natriuretic peptide; 3-30; 5-31; 3-32; 4-29

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4879 Human NPPB

Swiss-port # P16860 Human Natriuretic peptides B

Gene Symbol NPPB
Gene Full Name natriuretic peptide B
Background This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]
Function Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Signaling Transduction antibody
PTM The brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

Images (4) Click the Picture to Zoom In

  • ARG10146 anti-BNP antibody [50E1] WB image

    Western blot: Human synthetic BNP stained with ARG10146 anti-BNP antibody [50E1].

  • ARG10146 anti-BNP antibody [50E1] FLISA image

    FLISA: Biotinylated ARG10146 anti-BNP antibody [50E1] used as capture antibody and Eu3+ - labeled ARG10609 anti-BNP antibody [24C5] used as detection antibody. Mixture of antibodies (50 µl) and synthetic BNP (50 µl) is incubated for 30 min at RT in streptavidincoated plate.

  • ARG10146 anti-BNP antibody [50E1] stability studies image

    Stability studies: Recombinant glycosylated proBNP (from mammalian cell line) (green) and endogenous proBNP, purified from pooled HF patients plasma (red) were reconstituted in pooled normal human EDTA-plasma and incubated at RT for different time periods.
    Immunological activity was measured by immunoassay, utilizing ARG10146 anti-BNP antibody [50E1] as capture and ARG10609 anti-BNP antibody [24C5] as detection.

  • ARG10146 anti-BNP antibody [50E1] WB image

    Western blot: Recombinant proBNP (E. coli expressed) stained with ARG10146 anti-BNP antibody [50E1].

Clone References

Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes.

WB, ICC/IF / Human

Tonne JM et al.
Clin Chem.,  (2011)

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