ARG10833
anti-BPL / BioID2 antibody [SS 3A5-E2]
anti-BPL / BioID2 antibody [SS 3A5-E2] for ICC/IF,Western blot and Bacteria
Overview
| Product Description | Mouse Monoclonal antibody [SS 3A5-E2] recognizes BPL / BioID2 |
|---|---|
| Tested Reactivity | Bacteria |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SS 3A5-E2 |
| Isotype | IgG1, kappa |
| Target Name | BPL / BioID2 |
| Antigen Species | Bacteria |
| Immunogen | GST fused to A. aeolicus BPL R40G (BioID2). |
| Conjugation | Un-conjugated |
| Alternate Names | BPL; BioID2; BioID |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Cells overexpressing A. aeolicus BPL R40G (BioID2) construct. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Calculated MW | 27 kDa |
|---|
Images (3) Click the Picture to Zoom In
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ARG10833 anti-BPL/BioID2 antibody [SS 3A5-E2] ICC/IF image
Immunofluorescence: HeLa polyclonal cell line expressing BioID2 tagged to TorsinA ΔE302/3 and stained with ARG10833 anti-BPL/BioID2 antibody [SS 3A5-E2]. Cells were fixed in (A) cold methanol or (B) 4% PFA. Left: Primary antibodies (green). Middle: Hoechst (blue). Right: Merge.
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ARG10833 anti-BPL/BioID2 antibody [SS 3A5-E2] WB image
Western blot: Total cell lysate of parental HeLa cell line (Lane 1), HeLa polyclonal cell line stably expressing BioID2-TorsinA ΔE302/3 without (Lane 2) or with induced expression (Lane 3). The blots were stained with ARG10833 anti-BPL/BioID2 antibody [SS 3A5-E2].
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ARG10833 anti-BPL/BioID2 antibody [SS 3A5-E2] ICC/IF image
Immunofluorescence: (A) Parental HEK293T or (B) monoclonal HEK293T cell line with BioID2 "knocked-in" into at least one LMNA gene alleles (resulting in BioID2 LaminA/C fusion protein) were stained with ARG10833 anti-BPL/BioID2 antibody [SS 3A5-E2]. Left: Primary antibodies (green). Middle: Hoechst (blue). Right: Merge.
