ARG45584
anti-BRD4 antibody
anti-BRD4 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes BRD4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BRD4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to middle region of human BRD4. |
| Conjugation | Un-conjugated |
| Alternate Names | BRD4; Bromodomain Containing 4; HUNK1; FSHRG4; HUNKI; MCAP; CAP; Female Sterile Homeotic Related Gene 4; Mitotic Chromosome-Associated Protein; Bromodomain-Containing Protein 4; Chromosome-Associated Protein; Bromodomain-Containing 4; Protein HUNK1; CDLS6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 220 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BRD4 |
| Gene Full Name | Bromodomain Containing 4 |
| Background | The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| Function | Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. [UniProt] |
| Cellular Localization | Chromosome; Nucleus. [UniProt] |
| Calculated MW | 152 kDa |
| PTM | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45584 anti-BRD4 antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45584 anti-BRD4 antibody at 5 μg/ml dilution.
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ARG45584 anti-BRD4 antibody WB image
Western blot: HepG2 stained with ARG45584 anti-BRD4 antibody at 0.5 μg/ml dilution.
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ARG45584 anti-BRD4 antibody WB image
Western blot: NIH/3T3 stained with ARG45584 anti-BRD4 antibody at 0.5 μg/ml dilution.
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ARG45584 anti-BRD4 antibody WB image
Western blot: C6 stained with ARG45584 anti-BRD4 antibody at 0.5 μg/ml dilution.
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ARG45584 anti-BRD4 antibody FACS image
Flow Cytometry: A549 stained with ARG45584 anti-BRD4 antibody at 1 µg/10^6 cells dilution.
