anti-Bad antibody for ELISA,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
|Product Description||Rabbit Polyclonal antibody recognizes Bad|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ELISA, ICC/IF, IHC, WB|
|Immunogen||Synthetic peptide (15 aa) within aa. 90-140 of Human Bad protein.|
|Alternate Names||Bcl-2-binding component 6; Bcl-2-like protein 8; BCL2L8; Bcl-xL/Bcl-2-associated death promoter; BAD; Bcl2-associated agonist of cell death; BBC2; Bcl2 antagonist of cell death; Bcl2-L-8|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Positive Control||T24 Cell Lysate|
|Purification||Ion exchange chromatography.|
|Buffer||PBS and 0.02% Sodium azide|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||BCL2-associated agonist of cell death|
|Background||Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival, whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.|
|Function||Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. [UniProt]|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody|
|Calculated MW||18 kDa|
|PTM||Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.
Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.
Images (3) Click the Picture to Zoom In
ARG54681 anti-BAD antibody ICC/IF image
Immunofluorescence: rat thymus cells stained with ARG54681 anti-BAD antibody at 10 μg/ml.
ARG54681 anti-BAD antibody IHC image
Immunohistochemical: rat thymus stained with ARG54681 anti-Bad at 2 μg/ml.
ARG54681 anti-BAD antibody WB image
Western blot: T24 cell lysates stained with ARG54681 anti-Bad antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.