anti-Bad phospho (Ser155) antibody
anti-Bad phospho (Ser155) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
|Product Description||Rabbit Polyclonal antibody recognizes Bad phospho (Ser155)|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||IHC-P, WB|
|Immunogen||Peptide sequence around phosphorylation site of serine 155(R-M-S(p)-D-E) derived from Human BAD.|
|Alternate Names||Bcl-2-binding component 6; Bcl-2-like protein 8; BCL2L8; Bcl-xL/Bcl-2-associated death promoter; BAD; Bcl2-associated agonist of cell death; BBC2; Bcl2 antagonist of cell death; Bcl2-L-8|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.|
|Buffer||PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||BCL2-associated agonist of cell death|
|Background||Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.|
|Function||Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. [UniProt]|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody|
|Calculated MW||18 kDa|
|PTM||Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.
Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.
Images (2) Click the Picture to Zoom In
ARG51572 anti-Bad phospho (Ser155) antibody WB image
Western blot: Extracts from 293 cells stained with ARG51572 anti-Bad phospho (Ser155) antibody.
ARG51572 anti-Bad phospho (Ser155) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51572 anti-Bad phospho (Ser155) antibody.