ARG10552

anti-Bax antibody [G26-R]

anti-Bax antibody [G26-R] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description

Rabbit Monoclonal antibody [G26-R] recognizes Bax

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone G26-R
Isotype IgG
Target Name Bax
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human bax
Epitope Antibody recognizes the epitope located between Pro13 - Gly36.
Conjugation Un-conjugated
Full Name BCL2-associated X protein
Alternate Names Bcl-2-like protein 4; Bcl2-L-4; BCL2L4; Apoptosis regulator BAX

Application Instructions

Application Suggestion
Tested Application
ICC/IF1:100 – 1:600
IHC-P1:100
IP1:100 – 1:600
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 10 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12028 Mouse BAX

GeneID: 581 Human BAX

Swiss-port # Q07812 Human Apoptosis regulator BAX

Swiss-port # Q07813 Mouse Apoptosis regulator BAX

Gene Symbol BAX
Gene Full Name BCL2-associated X protein
Background The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
Function Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. [UniProt]

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  • ARG10552 anti-Bax antibody [G26-R] WB image

    Western blot: 20 µl of 293T, Mouse brain and Rat brain lysates stained with ARG10552 anti-Bax antibody [G26-R] at 1:1000 dilution.

  • ARG10552 anti-Bax antibody [G26-R] ICC/IF image

    Immunocytochemistry: HEK293 cells stained with ARG10552 anti-Bax antibody [G26-R] at 1:100 dilution.