ARG22186
anti-Bcl 2 antibody [10C4]
anti-Bcl 2 antibody [10C4] for Western blot,Immunoprecipitation,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [10C4] recognizes Bcl 2 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | Mouse/Rat Bcl-2 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 10C4 |
| Isotype | IgG1, kappa |
| Target Name | Bcl 2 |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide around aa. 61-76 of Murine Bcl-2 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Apoptosis regulator Bcl-2; Bcl-2; PPP1R50 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | BBS (pH 8.2) |
| Concentration | 0.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | BCL2 |
| Gene Full Name | B cell leukemia/lymphoma 2 |
| Background | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008] |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody |
| Calculated MW | 26 kDa |
| PTM | Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. |
