ARG54693

anti-Bcl 2 antibody

anti-Bcl 2 antibody for ELISA,Immunohistochemistry,Western blot,ICC/IF and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Bcl 2
Tested Reactivity Hu, Ms
Tested Application ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bcl 2
Antigen Species Human
Immunogen Synthetic peptide (15 aa) within the first 50 aa of Human Bcl-2.
Conjugation Un-conjugated
Alternate Names Apoptosis regulator Bcl-2; Bcl-2; PPP1R50

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF10 μg/ml
IHC2 μg/ml
WB4 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Daudi Cell Lysate

Properties

Form Liquid
Purification Ion exchange chromatography purified.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12043 Mouse BCL2

GeneID: 596 Human BCL2

Swiss-port # P10415 Human Apoptosis regulator Bcl-2

Swiss-port # P10417 Mouse Apoptosis regulator Bcl-2

Gene Symbol BCL2
Gene Full Name B-cell CLL/lymphoma 2
Background Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
Calculated MW 26 kDa
PTM Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Images (3) Click the Picture to Zoom In

  • ARG54693 anti-Bcl 2 antibody WB image

    Western blot: Daudi cell lysates stained with ARG54693 anti-Bcl 2 antibody at (A) 1, (B) 2, and (C) 4 ug/ml dilution.

  • ARG54693 anti-Bcl 2 antibody IHC image

    Immunohistochemistry: Human kidney stained with ARG54693 anti-Bcl 2 antibody at 2 ug/ml dilution.

  • ARG54693 anti-Bcl 2 antibody ICC/IF image

    Immunofluorescence: Human Kidney cells stained with ARG54693 anti-Bcl 2 antibody at 10 ug/ml dilution.