ARG54223

anti-Bcl 2 antibody [Bcl2/100/D5] (FITC)

anti-Bcl 2 antibody [Bcl2/100/D5] (FITC) for Flow cytometry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [Bcl-2/100/D5] recognizes Bcl 2
Tested Reactivity Hu
Species Does Not React With Ms
Tested Application FACS
Specificity The clone Bcl2/100 recognizes Bcl2, a 26 kDa protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
Host Mouse
Clonality Monoclonal
Clone Bcl2/100/D5
Isotype IgG1
Target Name Bcl 2
Antigen Species Human
Immunogen Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Conjugation FITC
Alternate Names Apoptosis regulator Bcl-2; Bcl-2; PPP1R50

Application Instructions

Application Suggestion
Tested Application Dilution
FACS4 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 596 Human BCL2

Swiss-port # P10415 Human Apoptosis regulator Bcl-2

Gene Symbol BCL2
Gene Full Name B-cell CLL/lymphoma 2
Background Bcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
Calculated MW 26 kDa
PTM Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Images (2) Click the Picture to Zoom In

  • ARG54223 anti-Bcl 2 antibody [Bcl2/100/D5] (FITC) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG54223 anti-Bcl 2 antibody [Bcl2/100/D5] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).

  • ARG54223 anti-Bcl 2 antibody [Bcl2/100/D5] (FITC) FACS image

    Flow Cytometry: Separation of human BCL2 positive lymphocytes (red-filled) from blood debris (black-dashed). Human peripheral whole blood stained with ARG54223 anti-Bcl 2 antibody [Bcl2/100/D5] (FITC) (4 µl reagent / 100 µl of peripheral whole blood).