ARG51568

anti-Bcl 2 phospho (Ser70) antibody

anti-Bcl 2 phospho (Ser70) antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Bcl 2 phospho (Ser70)
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bcl 2
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 70(R-T-S(p)-P-L) derived from Human Bcl-2.
Conjugation Un-conjugated
Alternate Names Apoptosis regulator Bcl-2; Bcl-2; PPP1R50

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 596 Human BCL2

Swiss-port # P10415 Human Apoptosis regulator Bcl-2

Gene Symbol BCL2
Gene Full Name B-cell CLL/lymphoma 2
Background Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody; Apoptosis Marker antibody
Calculated MW 26 kDa
PTM Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

Images (1) Click the Picture to Zoom In

  • ARG51568 anti-Bcl 2 phospho (Ser70) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma, stained with ARG51568 anti-Bcl 2 phospho (Ser70) antibody.