ARG21417

anti-Bcl XL antibody [7B2.5] (PE)

anti-Bcl XL antibody [7B2.5] (PE) for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Rhesus Monkey

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [7B2.5] recognizes Bcl XL
Tested Reactivity Hu, Ms, Rat, R. Mk
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity Human/Mouse/Rat/Rhesus Bcl-xL.
Host Mouse
Clonality Monoclonal
Clone 7B2.5
Isotype IgG3, kappa
Target Name Bcl XL
Antigen Species Human
Immunogen Recombinant human Bcl-xS
Conjugation PE
Full Name BCL2-like 1
Alternate Names Apoptosis regulator Bcl-X; BCLXS; BCL-XL/S; PPP1R52; bcl-xS; Bcl-2-like protein 1; Bcl2-L-1; Bcl-X; BCLX; bcl-xL; BCL2L; BCLXL

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 0.3 µg/10^6 cells
ICC/IFAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 26 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12048 Mouse BCL2L1

GeneID: 24888 Rat BCL2L1

GeneID: 598 Human BCL2L1

Gene Symbol BCL2L1
Gene Full Name BCL2-like 1
Background The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]
Function Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.

Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles.

Isoform Bcl-X(S) promotes apoptosis. [UniProt]
PTM Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis.

Images (1) Click the Picture to Zoom In

  • ARG21417 anti-Bcl XL antibody [7B2.5] (PE) FACS image

    Flow Cytometry: Human T cell leukemia cell line Jurkat was intracellularly stained with ARG21417 anti-Bcl XL antibody [7B2.5] (PE).