anti-Beclin 1 phospho (Ser295) antibody
anti-Beclin 1 phospho (Ser295) antibody for Western blot and Human
Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody
|Product Description||Rabbit Polyclonal antibody recognizes Beclin 1 phospho (Ser295)|
|Predict Reactivity||Ms, Bov, Chk, Dog, NHuPrm, Sheep, Xenopus laevis, Zfsh|
|Target Name||Beclin 1|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 conjugated to KLH|
|Alternate Names||VPS30; Beclin-1; beclin1; Coiled-coil myosin-like BCL2-interacting protein; ATG6; Protein GT197|
|Application Note||Specific for the ~60k beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||beclin 1, autophagy related|
|Background||Beclin 1 regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]|
|Function||Beclin 1 plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. [UniProt]
|Highlight||Related Antibody Duos and Panels:
ARG30094 Phospho Beclin Antibody Duo (pS234, pS295)
Beclin 1 antibodies; Beclin 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Keap1-Nrf2-ARE antibody panel is launched
Therapeutic strategies against PDAC
|Research Area||Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody|
|Calculated MW||52 kDa|
|PTM||Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy (PubMed:19180116). In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy (PubMed:23878393).
Polyubiquitinated by NEDD4, both with 'Lys11'- and 'Lys63'-linkages. 'Lys'-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.
Proteolytically processed by caspases including CASP8 and CASP3; the C-terminal fragments lack autophagy-inducing capacity and are proposed to induce apoptosis. Thus the cleavage is proposed to be an determinant to switch from autophagy to apoptosis pathways affecting cellular homeostasis including viral infections and survival of tumor cells.
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ARG52235 anti-Beclin 1 phospho (Ser295) antibody WB image
Western blot: beclin-1 expressed stained with ARG52235 anti-Beclin 1 phospho (Ser295) antibody at 1: 250 dilution in HeLa cells showing specific immunolabeling of beclin-1 in wild-type (WT), in addition, this immunolabeling is eliminated in the beclin-1 alanine substitution mutant (S295A) samples.