ARG10776

anti-Bestrophin antibody

anti-Bestrophin antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot,Dot blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Bestrophin
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bestrophin
Species Human
Immunogen Synthetic peptide from Human Bestrophin.
Conjugation Un-conjugated
Full Name bestrophin 1
Alternate Names Vitelliform macular dystrophy protein 2; RP50; BMD; TU15B; VMD2; Bestrophin-1; ARB; BEST

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:100
Dot1:10000
ELISA1:10000
ICC/IF1:100
IHC1:100
IP1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 68 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 24115 Mouse BEST1

GeneID: 7439 Human BEST1

Swiss-port # O76090 Human Bestrophin-1

Swiss-port # O88870 Mouse Bestrophin-1

Gene Symbol BEST1
Gene Full Name bestrophin 1
Background This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]
Function Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate. [UniProt]
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Images (2) Click the Picture to Zoom In

  • ARG10776 anti-Bestrophin antibody ICC/IF image

    Immunofluorescence: Basolateral cells stained with ARG10776 anti-Bestrophin antibody (green) at 1:100 dilution. DAPI (blue) for nuclear staining.

  • ARG10776 anti-Bestrophin antibody WB image

    Western blot: Recombinant Bestrophin protein stained with ARG10776 anti-Bestrophin antibody at 1:500 dilution.