ARG20530

anti-BiP / GRP78 antibody

anti-BiP / GRP78 antibody for Western blot,Immunohistochemistry,ICC/IF,ELISA and Dog,Human,Mouse,Rat,D. melanogaster

Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes BiP / GRP78

Tested Reactivity Hu, Ms, Rat, Dm, Dog
Predict Reactivity Rat
Tested Application ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Target Name BiP / GRP78
Species Human
Immunogen Full length of Human BiP / GRP78 with His tagged at the N-terminus.
Conjugation Un-conjugated
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Alternate Names Heat shock 70 kDa protein 5; BiP; GRP-78; Endoplasmic reticulum lumenal Ca; MIF2; 78 kDa glucose-regulated protein; GRP78; Immunoglobulin heavy chain-binding protein; HEL-S-89n; BIP; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1:100
IHCAssay-dependent
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 72 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14828 Mouse HSPA5

GeneID: 25617 Rat HSPA5

GeneID: 3309 Human HSPA5

Gene Symbol HSPA5
Gene Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Background The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
Function Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. [UniProt]
Research Area Controls and Markers antibody; ER Marker antibody; Endoplasmic reticulum antibody

Images (2) Click the Picture to Zoom In

  • ARG20530 anti-BiP / GRP78 antibody ICC/IF image

    Immunofluorescence: Heat Shocked (42°C for 1 hour) HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20530 anti-BiP / GRP78 antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20530 anti-BiP / GRP78 antibody. Right: Composite.

  • ARG20530 anti-BiP / GRP78 antibody WB image

    Western blot: Glia GD cell lysates stained with ARG20530 anti-BiP / GRP78 antibody at 1:1000 dilution.