ARG20055

anti-Bid antibody

anti-Bid antibody for Immunohistochemistry,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
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anti-Bid antibody

Rabbit Polyclonal antibody recognizes Bid

ARG20055 Arigo biolaboratories
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anti-Bid antibody is rated 5.0 out of 5 by 1.

anti-Bid antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes Bid

Tested Reactivity Hu
Tested Application IHC, IP, WB
Specificity The antibody detects 22 kDa Human Bid.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bid
Antigen Species Human
Immunogen Synthetic peptide surrounding aa. 37 of Human Bid.
Conjugation Un-conjugated
Alternate Names p22 BID; BID; p15 BID; p11 BID; BH3-interacting domain death agonist; p13 BID; FP497

Application Instructions

Application Suggestion
Tested Application Dilution
IHC20-40 µg/ml
IP5-10 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 22 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 637 Human BID

Swiss-port # P55957 Human BH3-interacting domain death agonist

Background Bid, a BH3 domain-containing proapoptotic Bcl-2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies, Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
Highlight Related Antibody Duos and Panels:
ARG30269 Apoptosis Marker Antibody Duo (Bcl2, Bid)
Related products:
Bid antibodies; Bid Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
PTM TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

Images (1) Click the Picture to Zoom In

  • ARG20055 anti-Bid antibody WB image

    Western blot: 30 µg of Jurkat cell lysate stained with ARG20055 anti-Bid antibody at 1:500 dilution.

Customer's Feedback

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Review for anti-Bid antibody

Application:WB

Sample:Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC