anti-Bmi 1 antibody

anti-Bmi 1 antibody for ICC/IF,Western blot and Human,Mouse

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Product Description Rabbit Polyclonal antibody recognizes Bmi 1
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bmi 1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 281-314 (C-terminus) of Human Bmi 1.
Conjugation Un-conjugated
Alternate Names Polycomb complex protein BMI-1; RING finger protein 51; Polycomb group RING finger protein 4

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Gene Symbol COMMD3-BMI1
Gene Full Name COMMD3-BMI1 readthrough
Background This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]
Function Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. [UniProt]
Cellular Localization Nucleus. Cytoplasm.
Calculated MW 37 kDa
PTM Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

Images (2) Click the Picture to Zoom In

  • ARG55885 anti-Bmi 1 antibody WB image

    Western blot: 30 µg of HeLa and A549 cell lysates stained with ARG55885 anti-Bmi 1 antibody at 1:1000 dilution.

  • ARG55885 anti-Bmi 1 antibody ICC/IF image

    Immunofluorescence: 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG55885 anti-Bmi 1 antibody at 1:25 dilution (green). Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (red) at 1:100 dilution.

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Sample:HeLa and A549

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.

WB / Human

Yang Yan et al.
Cancer Cell Int.,  (2020)