anti-Bnip 3L antibody

anti-Bnip 3L antibody for Immunohistochemistry,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody


Product Description Rabbit Polyclonal antibody recognizes Bnip 3L
Tested Reactivity Hu, Ms
Predict Reactivity Bov, Rat
Tested Application IHC, WB
Specificity This antibody recognizes monomeric human and mouse Bnip3L. A band of 40kDa is detected in immunoblots.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Bnip 3L
Antigen Species Human
Immunogen Peptide corresponding to aa 77-92 of human Bnip3L (accession no. AF067396) This amino acid sequence is identical to that of mouse.
Conjugation Un-conjugated
Alternate Names NIP3L; BNIP3a; Adenovirus E1B19K-binding protein B5; NIP3-like protein X; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; NIX; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like

Application Instructions

Application Suggestion
Tested Application Dilution
IHC0.5 - 2 μg/mL
WB2 - 10 μg/mL
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562 and Human kidney


Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 12177 Mouse BNIP3L

GeneID: 665 Human BNIP3L

Swiss-port # O60238 Human BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like

Swiss-port # Q9Z2F7 Mouse BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like

Gene Symbol BNIP3L
Gene Full Name BCL2/adenovirus E1B 19kDa interacting protein 3-like
Background A novel BH3 domain-containing protein was recently identified and designated Bnip3L, Bnip3 , or Nix. Bnip3L is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppression by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic-mitochondrial proteins.
Function Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 24 kDa
PTM Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin.

Images (2) Click the Picture to Zoom In

  • ARG54385 anti-Bnip 3L antibody WB image

    Western blot: K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide stained with ARG54385 anti-Bnip 3L antibody at 1 μg/ml dilution.

  • ARG54385 anti-Bnip 3L antibody IHC image

    Immunohistochemistry: Human kidney tissue stained with ARG54385 anti-Bnip 3L antibody at 2 μg/ml dilution.