ARG57809

anti-CA9 / Carbonic Anhydrase 9 antibody

anti-CA9 / Carbonic Anhydrase 9 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CA9 / Carbonic Anhydrase 9
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CA9 / Carbonic Anhydrase 9
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 52-151 of Human CA9 (NP_001207.2).
Conjugation Un-conjugated
Full Name carbonic anhydrase IX
Alternate Names EC 4.2.1.1; MN; CAIX; pMW1; Carbonic anhydrase 9; Carbonic anhydrase IX; RCC-associated antigen G250; Membrane antigen MN; P54/58N; Carbonate dehydratase IX; Renal cell carcinoma-associated antigen G250; CA-IX

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse small intestine

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 768 Human CA9

Swiss-port # Q16790 Human Carbonic anhydrase 9

Gene Symbol CA9
Gene Full Name carbonic anhydrase IX
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
Function Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. [UniProt]
Cellular Localization Cell membrane, Cell projection, Nucleus, Single-pass type I membrane protein, microvillus membrane, nucleolus. [UniProt]
Highlight Related products:
Carbonic Anhydrase9 antibodies; Carbonic Anhydrase9 ELISA Kits; Anti-Rabbit IgG secondary antibodies;
Related news:
Hypoxia-induced transcription, histone demethylases are involved
PTM Asn-346 bears high-mannose type glycan structures. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG57809 anti-CA9 / Carbonic Anhydrase 9 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG57809 anti-CA9 / Carbonic Anhydrase 9 antibody at 1:100 dilution.

  • ARG57809 anti-CA9 / Carbonic Anhydrase 9 antibody WB image

    Western blot: 25 µg of Mouse small intestine lysate stained with ARG57809 anti-CA9 / Carbonic Anhydrase 9 antibody at 1:1000 dilution.