ARG45461
anti-CAMKIV antibody
anti-CAMKIV antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CAMKIV |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CAMKIV |
| Antigen Species | Mouse |
| Immunogen | Recombinant protein containing to mouse CAMKIV. |
| Conjugation | Un-conjugated |
| Alternate Names | CAMK4; CaMK IV; CaMK-GR; EC 2.7.11.17; Calcium/calmodulin-dependent protein kinase type IV; caMK; IV; CaM kinase-GR |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 58 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CAMK4 |
|---|---|
| Gene Full Name | calcium/calmodulin-dependent protein kinase IV |
| Background | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consodilation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteolclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphrylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18. |
| Function | Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4(+)/CD8(+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 52 kDa |
| PTM | Phosphorylated by CaMKK1 and CaMKK2 on Thr-200. Dephosphorylated by protein phosphatase 2A. Autophosphorylated on Ser-12 and Ser-13. Glycosylation at Ser-189 modulates the phosphorylation of CaMK4 at Thr-200 and negatively regulates its activity toward CREB1 in basal conditions and during early inomycin stimulation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45461 anti-CAMKIV antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45461 anti-CAMKIV antibody at 2 μg/ml dilution.
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ARG45461 anti-CAMKIV antibody WB image
Western blot: Rat brain stained with ARG45461 anti-CAMKIV antibody at 0.25 μg/ml dilution.
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ARG45461 anti-CAMKIV antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45461 anti-CAMKIV antibody at 2 μg/ml dilution.
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ARG45461 anti-CAMKIV antibody ICC/IF image
Immunofluorescence: RM-1 stained with ARG45461 anti-CAMKIV antibody at 5 μg/ml dilution.
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ARG45461 anti-CAMKIV antibody WB image
Western blot: Mouse brain stained with ARG45461 anti-CAMKIV antibody at 0.25 μg/ml dilution.
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ARG45461 anti-CAMKIV antibody FACS image
Flow Cytometry: RAW264.7 stained with ARG45461 anti-CAMKIV antibody at 1 µg/10^6 cells dilution.
