ARG46820

anti-CAPN3 antibody

anti-CAPN3 antibody for Flow cytometry,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CAPN3
Tested Reactivity Hu, Ms
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CAPN3
Antigen Species Human
Immunogen E.coli-derived human CAPN3 recombinant protein (Position: I42-D764). Human CAPN3 shares 93.5% and 94.2% amino acid (aa) sequence identity with mouse and rat CAPN3, respectively.
Conjugation Un-conjugated
Alternate Names CANP3; CANPL3; NCL1; Calpain-3; Calcium-activated neutral proteinase 3; CANP 3; Calpain L3; Calpain p94; Muscle-specific calcium-activated neutral protease 3; New calpain 1; nCL-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WB0.25 - 0.5 ug/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 99 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen
Purification Note 4% Trehalose, 0.9% NaCl, 0.2% Na2HPO4
Buffer 4% Trehalose
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12335 Mouse CAPN3

GeneID: 825 Human CAPN3

Swiss-port # P20807 Human Calpain-3

Swiss-port # Q64691 Mouse Calpain-3

Gene Symbol CAPN3
Gene Full Name Calpain 3
Background Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008]
Function Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53. [UniProt]
Cellular Localization Cytoplasm; Nucleus. [UniProt]