ARG55069

anti-CBF beta antibody

anti-CBF beta antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,ICC/IF and Human,Mouse

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CBF beta
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CBF beta
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 61-90 (Center) of Human CBF beta.
Conjugation Un-conjugated
Alternate Names PEBP2-beta; Polyomavirus enhancer-binding protein 2 beta subunit; CBF-beta; SL3-3 enhancer factor 1 subunit beta; PEA2-beta; PEBP2B; SL3/AKV core-binding factor beta subunit; Core-binding factor subunit beta

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12400 Mouse CBFB

GeneID: 865 Human CBFB

Swiss-port # Q08024 Mouse Core-binding factor subunit beta

Swiss-port # Q13951 Human Core-binding factor subunit beta

Gene Symbol CBFB
Gene Full Name core-binding factor, beta subunit
Background The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. [UniProt]
Cellular Localization Nucleus.
Research Area Gene Regulation antibody
Calculated MW 22 kDa

Images (3) Click the Picture to Zoom In

  • ARG55069 anti-CBF beta antibody ICC/IF image

    Immunofluorescence: 293 cells stained with ARG55069 anti-CBF beta antibody.

  • ARG55069 anti-CBF beta antibody WB image

    Western blot: 35 µg of K562 cell lysate stained with ARG55069 anti-CBF beta antibody.

  • ARG55069 anti-CBF beta antibody FACS image

    Flow Cytometry: HL-60 cells stained with ARG55069 anti-CBF beta antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.