ARG53997

anti-CBX3 / HP1 gamma antibody

anti-CBX3 / HP1 gamma antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Hamster,Human,Monkey,Mouse,Rat

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody recognizes CBX3 / HP1 gamma
Tested Reactivity Hu, Ms, Rat, Hm, Mk
Tested Application ICC/IF, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name CBX3 / HP1 gamma
Antigen Species Human
Immunogen Purified recombinant Human HP1 gamma protein fragments.
Conjugation Un-conjugated
Alternate Names HP1-GAMMA; Chromobox protein homolog 3; HP1Hs-gamma; HP1 gamma; HECH; Heterochromatin protein 1 homolog gamma; Modifier 2 protein

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200
IHC-P1:200
IPAssay-dependent
WB1:1000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 22 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 0.75 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11335 Human CBX3

Swiss-port # Q13185 Human Chromobox protein homolog 3

Gene Symbol CBX3
Gene Full Name chromobox homolog 3
Background Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Function Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the mehtyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. [UniProt]
Cellular Localization Nucleus
Highlight Related Antibody Duos and Panels:
ARG30341 Heterochromatin Foci Antibody Duo
Related products:
CBX3 antibodies; CBX3 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Senescence Marker Antibody Panel is launched
Research Area Gene Regulation antibody
Calculated MW 21 kDa
PTM Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

Images (4) Click the Picture to Zoom In

  • ARG53997 anti-CBX3 / HP1 gamma antibody ICC/IF image

    Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG53997 anti-CBX3 / HP1 gamma antibody at 1:200 dilution.

  • ARG53997 anti-CBX3 / HP1 gamma antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded prostate cancer tissue stained with ARG53997 anti-CBX3 / HP1 gamma antibody at 1:200 dilution. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0).

  • ARG53997 anti-CBX3 / HP1 gamma antibody WB image

    Western blot: 3T3 cell lysate stained with ARG53997 anti-CBX3 / HP1 gamma antibody at 1:1000 dilution.

  • ARG53997 anti-CBX3 / HP1 gamma antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG53997 anti-CBX3 / HP1 gamma antibody.