anti-CCL2 / MCP1 antibody [S101]

anti-CCL2 / MCP1 antibody [S101] for ELISA,Neutralizing,Western blot and Human,Rabbit

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody


Product Description Mouse Monoclonal antibody [S101] recognizes CCL2 / MCP1
Tested Reactivity Hu, Rb
Tested Application ELISA, Neut, WB
Specificity Does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1β (IL-1β), serum amyloid A (SAA) and epidermal growth factor (EGF).
Host Mouse
Clonality Monoclonal
Clone S101
Isotype IgG1, kappa
Target Name CCL2 / MCP1
Antigen Species Human
Immunogen Purified recombinant Human CCL2 / MCP1.
Conjugation Un-conjugated
Alternate Names MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

Application Instructions

Application Note ELISA: The antibody can be used for quantitative detection of human MCP-1 in sandwich ELISA in combination with capture antibody S14.

Western Blot: 0.1 μg/lane of recombinant human MCP-1 can be detected when antibody is used at 0.1-1μg/mL.

Neutralizing: The antibody at a concentration of 0.5μg/mL, neutralized 1nM recombinant human MCP-1 induced monocyte chemotaxis in blind well chemotaxis chambers.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 100009130 Rabbit CCL2

GeneID: 6347 Human CCL2

Swiss-port # P13500 Human C-C motif chemokine 2

Swiss-port # P28292 Rabbit C-C motif chemokine 2

Gene Symbol CCL2
Gene Full Name chemokine (C-C motif) ligand 2
Background Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.
Function Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]
Highlight Related products:
MCP1 antibodies; MCP1 ELISA Kits; MCP1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody
Calculated MW 11 kDa
PTM Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.