ARG10040

anti-CCL2 / MCP1 antibody [S101] (Biotin)

anti-CCL2 / MCP1 antibody [S101] (Biotin) for ELISA and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody

Overview

Product Description

Biotin-conjugated Mouse Monoclonal antibody [S101] recognizes CCL2 / MCP1

Tested Reactivity Hu
Tested Application ELISA
Specificity Does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1β (IL-1β), serum amyloid A (SAA) and epidermal growth factor (EGF).
Host Mouse
Clonality Monoclonal
Clone S101
Isotype IgG1, kappa
Target Name CCL2 / MCP1
Antigen Species Human
Immunogen Purified recombinant Human CCL2 / MCP1.
Conjugation Biotin
Alternate Names MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

Application Instructions

Application Note ELISA: In combination with capture antibody S14 (Cat. No.: ARG10008) and avidin-HRP conjugate, this biotin conjugated antibody can be used as tracer for detection of human MCP-1 in sandwich ELISA.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2), 1% Gelatin and 0.1% Proclin-300
Preservative 0.1% Proclin-300
Stabilizer 1% Gelatin
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6347 Human CCL2

Swiss-port # P13500 Human C-C motif chemokine 2

Gene Symbol CCL2
Gene Full Name chemokine (C-C motif) ligand 2
Background This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Function Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]
Resrarch Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody
PTM Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.