anti-CCL2 / MCP1 antibody [S14]
anti-CCL2 / MCP1 antibody [S14] for ELISA,Neutralizing,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody
|Product Description||Mouse Monoclonal antibody [S14] recognizes CCL2 / MCP1|
|Tested Application||ELISA, Neut, WB|
|Specificity||Does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1β (IL-1β), serum amyloid A (SAA) and epidermal growth factor (EGF).|
|Target Name||CCL2 / MCP1|
|Immunogen||Purified recombinant Human CCL2 / MCP1.|
|Full Name||chemokine (C-C motif) ligand 2|
|Alternate Names||MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1|
|Application Note||ELISA: This antibody can be used as a capture antibody in sandwich ELISA for human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: ARG10196). Approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600pg/mL.
Neutralizing: The antibody (clone S14) at a concentration of 0.5μg/mL, neutralized 1nM recombinant human MCP-1 induced monocyte chemotaxis in blind well chemotaxis chambers. It was also found that S-14 could inhibit the activity of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1.
Western Blot: 0.1 μg/lane of recombinant human MCP-1 can be detected when antibody is used at 0.1-1μg/mL.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Protein G affinity purified|
|Buffer||0.01M PBS (pH 7.2)|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.|
|Function||Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]|
|Resrarch Area||Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody|
|PTM||Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.|