ARG10008

anti-CCL2 / MCP1 antibody [S14]

anti-CCL2 / MCP1 antibody [S14] for ELISA,Neutralizing,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody

Overview

Product Description Mouse Monoclonal antibody [S14] recognizes CCL2 / MCP1
Tested Reactivity Hu
Tested Application ELISA, Neut, WB
Specificity Does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1β (IL-1β), serum amyloid A (SAA) and epidermal growth factor (EGF).
Host Mouse
Clonality Monoclonal
Clone S14
Isotype IgG1, kappa
Target Name CCL2 / MCP1
Antigen Species Human
Immunogen Purified recombinant Human CCL2 / MCP1.
Conjugation Un-conjugated
Full Name chemokine (C-C motif) ligand 2
Alternate Names MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

Application Instructions

Application Note ELISA: This antibody can be used as a capture antibody in sandwich ELISA for human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: ARG10196). Approximately 1.5 pg/mL of MCP-1 in serum/plasma or 4pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600pg/mL.
Neutralizing: The antibody (clone S14) at a concentration of 0.5μg/mL, neutralized 1nM recombinant human MCP-1 induced monocyte chemotaxis in blind well chemotaxis chambers. It was also found that S-14 could inhibit the activity of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1.

Western Blot: 0.1 μg/lane of recombinant human MCP-1 can be detected when antibody is used at 0.1-1μg/mL.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6347 Human CCL2

Swiss-port # P13500 Human C-C motif chemokine 2

Gene Symbol CCL2
Background Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.
Function Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]
Resrarch Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody
PTM Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

Clone References

Tissue factor and monocyte chemoattractant protein-1 expression in hypertensive individuals with normal or increased carotid intima-media wall thickness.

Sardo MA et al.
Clin Chem.,  (2008)

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