anti-CD10 antibody [SQab1873]

anti-CD10 antibody [SQab1873] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human,Rat


Product Description Recombinant Rabbit Monoclonal antibody [SQab1873] recognizes CD10
Tested Reactivity Hu, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1873
Isotype IgG
Target Name CD10
Antigen Species Human
Immunogen Recombinant protein within aa. 1-500 of CD10.
Conjugation Un-conjugated
Alternate Names Enkephalinase; Neutral endopeptidase; Neutral endopeptidase 24.11; Common acute lymphocytic leukemia antigen; Neprilysin; CD antigen CD10; CALLA; EC; CD10; Skin fibroblast elastase; SFE; Atriopeptidase; NEP

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:800 - 1:2000
IHC-P1:3200 - 1:6400
WB1:2000 - 1:5000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 24590 Rat MME

GeneID: 4311 Human MME

Swiss-port # P07861 Rat Neprilysin

Swiss-port # P08473 Human Neprilysin

Gene Symbol MME
Gene Full Name membrane metallo-endopeptidase
Background CD10 is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017]
Function CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) (PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). [UniProt]
Highlight Related products:
CD10 antibodies; CD10 ELISA Kits; CD10 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
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Calculated MW 86 kDa
PTM Myristoylation is a determinant of membrane targeting.

Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG66325 anti-CD10 antibody [SQab1873] FACS image

    Flow Cytometry: Raji cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66325 anti-CD10 antibody [SQab1873] (red) at 1:2,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66325 anti-CD10 antibody [SQab1873] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded appendix tissue stained with ARG66325 anti-CD10 antibody [SQab1873] at 1:6,400 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66325 anti-CD10 antibody [SQab1873] WB image

    Western blot: 10 µg of Raji and Ramos cell lysates stained with ARG66325 anti-CD10 antibody [SQab1873] at 1:2000 dilution.

  • ARG66325 anti-CD10 antibody [SQab1873] WB image

    Western blot: 10 µg of LnCap and Rat kidney lysates stained with ARG66325 anti-CD10 antibody [SQab1873] at 1:2000 dilution.