ARG21104

anti-CD102 / ICAM2 antibody [3C4] (Biotin)

anti-CD102 / ICAM2 antibody [3C4] (Biotin) for Flow cytometry,IHC-Frozen sections,ICC/IF,Blocking and Mouse

Overview

Product Description Biotin-conjugated Rat Monoclonal antibody [3C4] recognizes CD102 / ICAM2
Tested Reactivity Ms
Tested Application BL, FACS, ICC/IF, IHC-Fr
Specificity Mouse CD102. The clone 3C4 reacts with the cell surface glycoprotein mouse CD102 (ICAM-2), a ligand for the leucocyte integrin CD11a/CD18 (LFA-1). CD102 is constitutively expressed on T cells, B cells, and at high levels on vascular endothelial cells. It is also expressed on a variety of leukocyte cell lines. The 3C4 antibody blocks the interaction between ICAM-2 and LFA-1.
Host Rat
Clonality Monoclonal
Clone 3C4
Isotype IgG2a, kappa
Target Name CD102 / ICAM2
Antigen Species Mouse
Immunogen COS cells transfected with mouse ICAM-2 cDNA and BW5147 AKR/J mouse thymoma cell line
Conjugation Biotin
Full Name intercellular adhesion molecule 2
Alternate Names CD102; CD antigen CD102; Intercellular adhesion molecule 2; ICAM-2

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 31 kDa

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 15896 Mouse ICAM2

Swiss-port # P35330 Mouse Intercellular adhesion molecule 2

Gene Symbol ICAM2
Gene Full Name intercellular adhesion molecule 2
Background The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. [UniProt]