ARG20962

anti-CD106 / VCAM1 antibody [1.G11B1]

anti-CD106 / VCAM1 antibody [1.G11B1] for Blocking,ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Pig

Overview

Product Description Mouse Monoclonal antibody [1.G11B1] recognizes CD106 / VCAM1
Tested Reactivity Hu, Pig
Tested Application BL, ELISA, FACS, ICC/IF, IHC-Fr, IP, WB
Specificity Human/Porcine CD106. The clone 1.G11B1 inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium.
Host Mouse
Clonality Monoclonal
Clone 1.G11B1
Isotype IgG1, kappa
Target Name CD106 / VCAM1
Species Human
Conjugation Un-conjugated
Full Name vascular cell adhesion molecule 1
Alternate Names CD106; INCAM-100; Vascular cell adhesion protein 1; VCAM-1; CD antigen CD106; V-CAM 1

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 81 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 7412 Human VCAM1

Swiss-port # P19320 Human Vascular cell adhesion protein 1

Gene Symbol VCAM1
Gene Full Name vascular cell adhesion molecule 1
Background This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
Function Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt]
PTM Sialoglycoprotein.

Images (1) Click the Picture to Zoom In

  • ARG20962 anti-CD106 / VCAM1 antibody [1.G11B1] FACS image

    Flow Cytometry: TNFα stimulated Human endothelial cell line HUV-EC-C was stained with ARG20962 anti-CD106 / VCAM1 antibody [1.G11B1] followed by ARG21884 Goat anti-Mouse IgG1 antibody (FITC) (pre-adsorbed).