ARG22265

anti-CD107b / LAMP2 antibody [GL2A7]

anti-CD107b / LAMP2 antibody [GL2A7] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunohistochemistry (PFA perfusion fixed frozen sections),Immunoprecipitation,Western blot and Human,Mouse,Rabbit

Overview

Product Description Rat Monoclonal antibody [GL2A7] recognizes CD107b / LAMP2
Tested Reactivity Hu, Ms, Rb
Tested Application FACS, ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, IP, WB
Specificity Detects ~100-110kDa.
Host Rat
Clonality Monoclonal
Clone GL2A7
Isotype IgG1
Target Name CD107b / LAMP2
Antigen Species Mouse
Immunogen Purified preparation of Mouse liver lysosomal membranes
Conjugation Un-conjugated
Alternate Names CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1µg for 10^6 cells
ICC/IF1:500
IHC-FoFr Assay-dependent
IHC-FrAssay-dependent
IHC-P1:100 - 1:500
IPAssay-dependent
WB1:1000
Application Note For best ICC/IF results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained.
For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 100-110 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16784 Mouse LAMP2

GeneID: 3920 Human LAMP2

Swiss-port # P13473 Human Lysosome-associated membrane glycoprotein 2

Swiss-port # P17047 Mouse Lysosome-associated membrane glycoprotein 2

Gene Symbol Lamp2
Gene Full Name lysosomal-associated membrane protein 2
Background The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]
Function Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. [UniProt]
Cellular Localization Cell membrane, Endosome, Endosome membrane, Lysosome, Lysosome membrane
Calculated MW 45 kDa
PTM O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

Images (1) Click the Picture to Zoom In

  • ARG22265 anti-CD107b / LAMP2 antibody [GL2A7] WB image

    Western blot: 1) MW ladder, 2) 20 µg of HEK293, and 3) 10 µg of NIH3T3 lysates stained with ARG22265 anti-CD107b / LAMP2 antibody [GL2A7] at 1:500 dilution (60 min at RT). Block: 5% milk + TBST 1hr at RT.