ARG54253

anti-CD107b / LAMP2 antibody [H4B4] (PE)

anti-CD107b / LAMP2 antibody [H4B4] (PE) for Electron microscopy,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Purification,Western blot and African green monkey,Human,Rhesus Monkey

Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [H4B4] recognizes CD107b / LAMP2
Tested Reactivity Hu, AGMK, R. Mk
Tested Application EM, FACS, ICC/IF, IHC-Fr, IHC-P, Puri, WB
Host Mouse
Clonality Monoclonal
Clone H4B4
Isotype IgG1, kappa
Target Name CD107b / LAMP2
Antigen Species Human
Immunogen Human adherent peripheral blood cells
Conjugation PE
Alternate Names CD antigen CD107b; LAMPB; CD107b; LGP110; Lysosome-associated membrane glycoprotein 2; LAMP-2; CD107 antigen-like family member B; Lysosome-associated membrane protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
EMAssay-dependent
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
PuriAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS, 0.1% Sodium azide and Sucrose.
Preservative 0.1% Sodium azide
Stabilizer Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3920 Human LAMP2

Swiss-port # P13473 Human Lysosome-associated membrane glycoprotein 2

Gene Symbol LAMP2
Gene Full Name lysosomal-associated membrane protein 2
Background CD107b (lysosome-associated membrane protein-2, LAMP-2), together with CD107a / LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, especially CD107b is important regulator in successful phagosomal maturation. CD107b deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danons disease). Immature CD107b is an approximately 45 kDa protein, but after extensive glycosylation the mature glycoprotein has about 100-120 kDa.
Function Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 45 kDa
PTM O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

Images (1) Click the Picture to Zoom In

  • ARG54253 anti-CD107b / LAMP2 antibody [H4B4] (PE) FACS image

    Flow Cytometry: Jurkat cells stained with ARG54253 anti-CD107b / LAMP2 antibody [H4B4] (PE).

Clone References

Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy.

WB, ICC/IF / Human

Mancias JD et al.
Nature.,  (2014)

publication_link

 

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Amino acids activate mammalian target of rapamycin (mTOR) complex 1 without changing Rag GTPase guanyl nucleotide charging.

Oshiro N et al.
J Biol Chem.,  (2014)

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Efficient intracerebral delivery of AAV5 vector encoding human ARSA in non-human primate.

IHC-FoFr / Macaque Monkey

Colle MA et al.
Hum Mol Genet.,  (2010)

publication_link

 

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Autophagy and exosomes in the aged retinal pigment epithelium: possible relevance to drusen formation and age-related macular degeneration.

FACS / Human

Wang AL et al.
PLoS One.,  (2009)

publication_link

 

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Identification of the SPG15 gene, encoding spastizin, as a frequent cause of complicated autosomal-recessive spastic paraplegia, including Kjellin syndrome.

ICC/IF / African Green Monkey

Hanein S et al.
Am J Hum Genet.,  (2008)

publication_link

 

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