ARG21337

anti-CD14 antibody [61D3] (PE)

anti-CD14 antibody [61D3] (PE) for Flow cytometry,IHC-Frozen sections,ICC/IF,Western blot,ELISA,Blocking and Human,Dog

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [61D3] recognizes CD14
Tested Reactivity Hu, Dog
Tested Application BL, ELISA, FACS, ICC/IF, IHC-Fr, WB
Specificity Human/Cynomolgus/Canine/Hooded Seal CD14.
Host Mouse
Clonality Monoclonal
Clone 61D3
Isotype IgG1, kappa
Target Name CD14
Antigen Species Human
Immunogen Human peripheral monocytes
Conjugation PE
Full Name CD14 molecule
Alternate Names CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 40 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 929 Human CD14

Swiss-port # P08571 Human Monocyte differentiation antigen CD14

Gene Symbol CD14
Gene Full Name CD14 molecule
Background The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
PTM N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

Images (1) Click the Picture to Zoom In

  • ARG21337 anti-CD14 antibody [61D3] (PE) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG21337 anti-CD14 antibody [61D3] (PE) and anti-CD3 antibody [UCHT1] (FITC).