anti-CD14 antibody [MEM-15] (PerCP)

anti-CD14 antibody [MEM-15] (PerCP) for Flow cytometry and Human,Primates

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody


Product Description

PerCP-conjugated Mouse Monoclonal antibody [MEM-15] recognizes CD14

Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.
MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
HLDA III; WS Code M 252
HLDA IV; WS Code M 113
HLDA IV; WS Code NL 90
HLDA IV; WS Code T 53
HLDA V; WS Code M MA086
Host Mouse
Clonality Monoclonal
Clone MEM-15
Isotype IgG1
Target Name CD14
Antigen Species Human
Immunogen A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Conjugation PerCP
Alternate Names CD antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; Monocyte differentiation antigen CD14

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Note The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 929 Human CD14

Swiss-port # P08571 Human Monocyte differentiation antigen CD14

Gene Symbol CD14
Gene Full Name CD14 molecule
Background CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Function In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Macrophages and neutrophils antibody
Calculated MW 40 kDa
PTM N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

Images (1) Click the Picture to Zoom In

  • ARG53769 anti-CD14 antibody [MEM-15] (PerCP) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG53769 anti-CD14 antibody [MEM-15] (PerCP).

Clone References

Targeting the giant cell tumor stromal cell: functional characterization and a novel therapeutic strategy.

FACS / Human

Steensma MR et al.
PLoS One.,  (2013)




Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression.

Sing A et al.
J Exp Med.,  (2002)




CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited.

Funda DP et al.
Infect Immun.,  (2001)




Soluble CD14 from urine copurifies with a potent inducer of cytokines.

Sundan A et al.
Eur J Immunol.,  (1994)