ARG21220

anti-CD146 antibody [C264]

anti-CD146 antibody [C264] for Flow cytometry,ICC/IF,Immunoprecipitation and Chicken,Turkey

Overview

Product Description Mouse Monoclonal antibody [C264] recognizes CD146
Tested Reactivity Chk, Turkey
Tested Application FACS, ICC/IF, IP
Specificity Chicken/Turkey MCAM.
Host Mouse
Clonality Monoclonal
Clone C264
Isotype IgG2b, kappa
Target Name CD146
Antigen Species Chicken
Immunogen Mixture of E15 and E16 thymocytes from H.B15, H.B19, and H.B21 chicken embryos
Conjugation Un-conjugated
Full Name melanoma cell adhesion molecule
Alternate Names Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; Cell surface glycoprotein MUC18; Melanoma cell adhesion molecule; CD146; MUC18; CD antigen CD146; S-endo 1 endothelial-associated antigen

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 1 µg/10^6 cells
ICC/IFAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 72 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol MCAM
Gene Full Name melanoma cell adhesion molecule
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21220 anti-CD146 antibody [C264] FACS image

    Flow Cytometry: Chicken thymocytes stained with ARG21220 anti-CD146 antibody [C264] followed by ARG21575 Goat anti-Mouse IgG2b antibody (PE) (pre-adsorbed).